1TGJ
HUMAN TRANSFORMING GROWTH FACTOR-BETA 3, CRYSTALLIZED FROM DIOXANE
Experimental procedure
Source type | ROTATING ANODE |
Source details | ENRAF-NONIUS FR571 |
Temperature [K] | 293 |
Detector technology | DIFFRACTOMETER |
Collection date | 1994-12-16 |
Detector | ENRAF-NONIUS FAST |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 49.300, 49.300, 78.900 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 7.000 - 2.000 |
R-factor | 0.175 |
Rwork | 0.175 |
Structure solution method | MR |
Starting model (for MR) | TGF-BETA2 (SCHLUNEGGER & GRUETTER) |
RMSD bond length | 0.070 |
RMSD bond angle | 24.890 * |
Data reduction software | ROTAVATA |
Data scaling software | CCP4 ((AGROVATA) |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 10.000 * | 2.100 |
High resolution limit [Å] | 2.000 * | 2.000 |
Rmerge | 0.070 | 0.353 |
Number of reflections | 6323 | |
Completeness [%] | 76.0 | 37.4 |
Redundancy | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 5 | 15% (V/V) DIOXANE, 100 MM SODIUM ACETATE, PH 5.0 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 3-6 (mg/ml) | |
2 | 1 | drop | acetic acid | 10 (mM) | |
3 | 1 | reservoir | dioxane | 15 (%(v/v)) | |
4 | 1 | reservoir | sodium acetate | 100 (mM) | pH5.0 |