1TFJ
Crystal structure of Bovine Glycolipid transfer protein in complex with a fatty acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-10-24 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.804 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.395, 34.904, 58.168 |
| Unit cell angles | 90.00, 116.16, 90.00 |
Refinement procedure
| Resolution | 18.630 - 1.610 |
| R-factor | 0.18405 |
| Rwork | 0.182 |
| R-free | 0.22141 |
| Structure solution method | MIR |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.669 |
| Data scaling software | XDS |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.700 |
| High resolution limit [Å] | 1.610 | 1.610 |
| Rmerge | 0.067 | 0.414 |
| Number of reflections | 25509 | |
| <I/σ(I)> | 12.16 | 3.27 |
| Completeness [%] | 97.1 | 95.1 |
| Redundancy | 4.1 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 280 | PEG 8000, MES, Sodium acetate, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 280K |
