1TFE
DIMERIZATION DOMAIN OF EF-TS FROM T. THERMOPHILUS
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Detector | FUJI |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 60.100, 60.100, 94.300 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 1.700 |
| R-factor | 0.207 |
| Rwork | 0.207 |
| R-free | 0.28500 |
| Structure solution method | MIR |
| RMSD bond length | 0.011 |
| RMSD bond angle | 22.490 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | DPHASE |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.068 | |
| Number of reflections | 24914 | |
| Completeness [%] | 93.5 | 88.6 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * | 18 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 55 (mM) | |
| 3 | 1 | drop | PEG3000 | 5 (%) | |
| 4 | 1 | drop | 20 (mM) | ||
| 5 | 1 | reservoir | PEG3000 | 10 (%) | |
| 6 | 1 | reservoir | 40 (mM) | ||
| 7 | 1 | reservoir | Tris-HCl | 100 (mM) |
