1TFA
OVOTRANSFERRIN, N-TERMINAL LOBE, APO FORM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | MACSCIENCE M18X |
| Temperature [K] | 293 |
| Detector technology | AREA DETECTOR |
| Collection date | 1997-07-15 |
| Detector | SIEMENS |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 125.170, 125.170, 87.260 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 7.000 - 1.900 |
| R-factor | 0.187 |
| Rwork | 0.187 |
| R-free | 0.24300 |
| Structure solution method | MIR |
| RMSD bond length | 0.012 |
| RMSD bond angle | 24.200 * |
| Data reduction software | SAINT |
| Data scaling software | SAINT |
| Phasing software | PHASES |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 1.830 |
| Number of reflections | 194748 |
| Completeness [%] | 95.5 |
| Redundancy | 5.62 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6 | 20 * | pH 6.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 44.4 (mg/ml) | |
| 2 | 1 | drop | Bis Tris-HCl | 0.05 (M) | |
| 3 | 1 | reservoir | Bis-Tris | 0.05 (M) | |
| 4 | 1 | reservoir | ammonium sulfate | 52 (%) |
