1TF7
Crystal Structure of Circadian Clock Protein KaiC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 5ID-B |
| Synchrotron site | APS |
| Beamline | 5ID-B |
| Temperature [K] | 108 |
| Detector technology | CCD |
| Collection date | 2002-07-10 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 132.873, 135.576, 204.951 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.800 |
| R-factor | 0.24 |
| Rwork | 0.240 |
| R-free | 0.28000 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.384 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | GLRF (MLPHARE RAVE) |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.043 | 0.501 |
| Number of reflections | 87750 | |
| <I/σ(I)> | 20.6 | 2.2 |
| Completeness [%] | 99.6 | 99.3 |
| Redundancy | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION, RECRYSTALLIZATION | 4 | 291 | sodium formate, glycerol, pH 4, EVAPORATION, RECRYSTALLIZATION, temperature 291K |
| 1 | EVAPORATION, RECRYSTALLIZATION | 4 | 291 | sodium formate, glycerol, pH 4, EVAPORATION, RECRYSTALLIZATION, temperature 291K |
