1TEL
Crystal structure of a RubisCO-like protein from Chlorobium tepidum
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9A |
| Synchrotron site | NSLS |
| Beamline | X9A |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2004-03-21 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97938, 0.97919, 0.96408 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.105, 77.708, 88.758 |
| Unit cell angles | 90.00, 99.57, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.700 |
| R-factor | 0.212 |
| Rwork | 0.209 |
| R-free | 0.24800 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.520 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 24718 | |
| Completeness [%] | 99.4 | 99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | PEG 3350, Tris HCl, magnesium chloride, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
