1TEH
STRUCTURE OF HUMAN LIVER CHICHI ALCOHOL DEHYDROGENASE (A GLUTATHIONE-DEPENDENT FORMALDEHYDE DEHYDROGENASE)
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-03-22 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 141.250, 201.550, 69.610 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.700 |
| R-factor | 0.206 |
| Rwork | 0.206 |
| R-free | 0.28300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 24.670 * |
| Data reduction software | PROCESS |
| Data scaling software | SCALE |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.700 | 2.750 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.095 | 0.250 |
| Total number of observations | 97001 * | |
| Number of reflections | 24896 | |
| <I/σ(I)> | 7.8 | 2.2 |
| Completeness [%] | 89.5 | 67 |
| Redundancy | 4 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 8 | 15 MG/ML ENZYME IN 280 MM MES, PH 6.7, 2 MM NAD+, 8.0 % PEG8000 AT 295 K, SITTING-DROP METHOD. |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | enzyme | 7.5-11 (mg/ml) | |
| 2 | 1 | reservoir | MES | 240 (mM) | |
| 3 | 1 | reservoir | NAD+ | 2.0 (mM) | |
| 4 | 1 | reservoir | 0.01 (M) | ||
| 5 | 1 | reservoir | PEG8000 | 8.0 (%(w/v)) |
