1TEG
Crystal structure of the spinach plastocyanin mutants G8D/K30C/T69C and K30C/T69C- a study of the effect on crystal packing and thermostability from the introduction of a novel disulfide bond
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-04-25 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.09500 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 52.196, 52.196, 126.928 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.200 - 1.960 |
| R-factor | 0.287 |
| Rwork | 0.287 |
| R-free | 0.30400 |
| Structure solution method | ISOMORPHOUS STRUCTURE |
| Starting model (for MR) | 1tef - G8D K30C T69C tripple mutant. |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.600 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.200 | 2.070 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Rmerge | 0.050 | 0.100 |
| Number of reflections | 14601 | |
| <I/σ(I)> | 34.15 | 6.97 |
| Completeness [%] | 98.1 | 90.69 |
| Redundancy | 10.83 | 6.86 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 21% PEG 3350, 0.25M MgCl2, 0.1M Na-acetate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
