1TE6

Crystal Structure of Human Neuron Specific Enolase at 1.8 angstrom

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2004-02-07
Detector	CUSTOM-MADE
Spacegroup name	P 21 21 2
Unit cell lengths	114.579, 119.708, 68.216
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.800
R-factor	0.206
Rwork	0.206
R-free	0.23400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2one
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	DENZO
Phasing software	CNS (1.1)
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.880
High resolution limit [Å]	1.800	1.800
Rmerge	0.077	0.077
Number of reflections	87702
Completeness [%]	99.6	97.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8.5	290	PEG 4000,magnesium chloride, Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K, pH 8.50