1TE2
Putative Phosphatase Ynic from Escherichia coli K12
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-18 |
| Detector | SBC-2 |
| Wavelength(s) | 0.9794, 0.9795, 0.956 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.591, 88.447, 48.591 |
| Unit cell angles | 90.00, 97.26, 90.00 |
Refinement procedure
| Resolution | 25.160 - 1.760 |
| R-factor | 0.1787 |
| Rwork | 0.174 |
| R-free | 0.21964 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0000) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.160 | 1.830 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Rmerge | 0.087 | 0.280 |
| Number of reflections | 38493 | |
| <I/σ(I)> | 6.9 | 3.95 |
| Completeness [%] | 96.0 | 71.2 |
| Redundancy | 5.9 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 298 | Calcium Chloride PEG 3350 or PEGMME 3350, Sodium cacodylate, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
