1TE0
Structural analysis of DegS, a stress sensor of the bacterial periplasm
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-04-09 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9393 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 166.280, 166.280, 166.280 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.620 - 2.200 |
| R-factor | 0.24711 |
| Rwork | 0.245 |
| R-free | 0.29526 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | DegP |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.413 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.000 | |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.048 | 0.390 |
| Number of reflections | 38296 | |
| <I/σ(I)> | 3 | |
| Completeness [%] | 98.6 | 98.6 |
| Redundancy | 5.4 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.05 M MgCl2, 0.1 M Hepes, pH 7.5, 30% Polyethylene Glycol Monomethyl ether 550, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
