1TDW
Crystal structure of double truncated human phenylalanine hydroxylase BH4-responsive PKU mutant A313T.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-D |
Temperature [K] | 277 |
Detector technology | IMAGE PLATE |
Collection date | 2003-03-12 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 66.411, 108.138, 124.620 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.930 - 2.100 |
R-factor | 0.206 |
Rwork | 0.206 |
R-free | 0.23700 |
Structure solution method | Difference fourier |
Starting model (for MR) | 1pah |
RMSD bond length | 0.013 |
RMSD bond angle | 1.500 |
Data reduction software | HKL-2000 |
Data scaling software | d*TREK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.170 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.118 | 0.612 |
Number of reflections | 26608 | |
<I/σ(I)> | 20 | 20 |
Completeness [%] | 100.0 | 100 |
Redundancy | 7.5 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 277 | 5-10% Ethylene Glycol, 20-40 mM Pipes, pH 6.8, 8-15% PEG 2000, VAPOR DIFFUSION, HANGING DROP, temperature 277K |