1TDT
THREE-DIMENSIONAL STRUCTURE OF TETRAHYDRODIPICOLINATE-N-SUCCINYLTRANSFERASE
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 293 |
Detector technology | AREA DETECTOR |
Collection date | 1996 |
Detector | SIEMENS-NICOLET X100 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 60.600, 107.900, 70.700 |
Unit cell angles | 90.00, 113.00, 90.00 |
Refinement procedure
Resolution | ? - 2.200 |
R-factor | 0.17 * |
Rwork | 0.170 |
R-free | 0.23200 * |
Structure solution method | MIR - 2 HEAVY ATOM DERIVATIVES |
RMSD bond length | 0.009 |
RMSD bond angle | 1.970 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASES |
Refinement software | TNT |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 2.300 | |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.067 | |
Total number of observations | 137056 * | |
Number of reflections | 38447 | |
Completeness [%] | 90.0 | 73 |
Redundancy | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 7.5 | 14-19 % (W/V) POLYETHYLENEGLYCOL 4000 200 MM AMMONIUM SULFATE 6-12 % 2-PROPANOL 100 MM HEPES, PH 7.5, |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | PEG4000 | 16 (%(w/v)) | |
2 | 1 | 1 | ammonium sulfate | 200 (mM) | |
3 | 1 | 1 | HEPES | 100 (mM) | |
4 | 1 | 1 | 2-propanol | 10 (%(v/v)) |