1TDQ

Structural basis for the interactions between tenascins and the C-type lectin domains from lecticans: evidence for a cross-linking role for tenascins

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I711
Synchrotron site	MAX II
Beamline	I711
Temperature [K]	100
Detector technology	CCD
Collection date	2003-02-01
Detector	MARRESEARCH
Wavelength(s)	1.097
Spacegroup name	P 21 21 2
Unit cell lengths	112.520, 86.420, 57.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.000 - 2.600
R-factor	0.20995
Rwork	0.209
R-free	0.23441
Structure solution method	MIRAS
RMSD bond length	0.012
RMSD bond angle	1.296
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	SHARP
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.000	2.710
High resolution limit [Å]	2.570	2.570
Number of reflections	18210
<I/σ(I)>	7.2	3.4
Completeness [%]	97.3	91.7
Redundancy	8.7	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	294	8-12% PEG 8000, 150mM calcium acetate, 2-4% PEG MME 750, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K