1TDH

Crystal structure of human endonuclease VIII-like 1 (NEIL1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2003-09-03
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	H 3
Unit cell lengths	132.190, 132.190, 51.090
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.100
Rwork	0.194
R-free	0.23100
Structure solution method	MIR
RMSD bond length	0.005
RMSD bond angle	1.289
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.230
High resolution limit [Å]	2.100	2.100
Rmerge	0.085	0.383
Number of reflections	19225
<I/σ(I)>	39.6	5.6
Completeness [%]	98.9	98.2
Redundancy	11.9	11.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	285	PEG 4000, tris, lithium sulfate, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 285K