1TD2
Crystal Structure of the PdxY Protein from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-01-29 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97900 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.130, 67.375, 73.589 |
| Unit cell angles | 90.00, 93.67, 90.00 |
Refinement procedure
| Resolution | 38.200 - 2.220 |
| R-factor | 0.176 |
| Rwork | 0.176 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lhr |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.600 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.290 |
| High resolution limit [Å] | 2.210 | 2.210 |
| Rmerge | 0.128 | 0.377 |
| Number of reflections | 28233 | |
| <I/σ(I)> | 11.9 | 4 |
| Completeness [%] | 90.3 | 89.1 |
| Redundancy | 2.4 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | ammonium sulfate, sodium-2-(N-morpholino)ethanesulfonate, potassium phosphate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
