1TD0
Viral capsid protein SHP at pH 5.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 3 |
| Unit cell lengths | 57.482, 57.482, 101.608 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.740 - 1.950 |
| R-factor | 0.229 |
| Rwork | 0.229 |
| R-free | 0.26000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.076 | 0.514 |
| Number of reflections | 27034 | |
| <I/σ(I)> | 2.6 | |
| Completeness [%] | 98.5 | 98.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 295 | sodium Acetate, PEG 5000 MME, MgCl2, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
