1TCM

CYCLODEXTRIN GLYCOSYLTRANSFERASE W616A MUTANT FROM BACILLUS CIRCULANS STRAIN 251

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1995-02-10
Detector	MAC Science DIP-2000
Spacegroup name	P 1 21 1
Unit cell lengths	73.700, 84.800, 118.300
Unit cell angles	90.00, 107.00, 90.00

Refinement procedure

Resolution	6.500 - 2.200
R-factor	0.193 *
Rwork	0.193
R-free	0.25000
RMSD bond length	0.005
RMSD bond angle	0.007 *
Data reduction software	XDS
Data scaling software	XDS
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.100 *
High resolution limit [Å]	2.200 *	2.200 *
Rmerge	0.083
Total number of observations	133691 *
Number of reflections	50563
Completeness [%]	71.3 *	51.5 *
Redundancy	2.64

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000	9-12 (%(w/v))
2	1	reservoir	sodium acetate	10 (mM)
3	1	drop	protein	8 (mg/ml)