1TCD
TRYPANOSOMA CRUZI TRIOSEPHOSPHATE ISOMERASE
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | MACSCIENCE |
| Temperature [K] | 295 |
| Detector technology | AREA DETECTOR |
| Collection date | 1996-10-02 |
| Detector | SIEMENS |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.710, 77.650, 149.540 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 1.830 |
| R-factor | 0.191 |
| Rwork | 0.191 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5tim |
| RMSD bond length | 0.013 |
| RMSD bond angle | 24.700 * |
| Data reduction software | SAINT |
| Data scaling software | SAINT ((SCALING ADAPTED FROM XSCALIBRE)) |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 1.930 | |
| High resolution limit [Å] | 1.830 | 1.830 |
| Rmerge | 0.109 | 0.109 |
| Total number of observations | 132483 * | |
| Number of reflections | 42089 | |
| <I/σ(I)> | 10 | 3 |
| Completeness [%] | 97.4 | 58.5 |
| Redundancy | 2.5 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | pH 7.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | reservoir | Na-Hepes | 0.1 (M) | |
| 3 | 1 | reservoir | PEG400 | 2 (%(v/v)) | |
| 4 | 1 | reservoir | ammonium sulfate | 2.0 (M) |
