1TCC

THE SEQUENCE, CRYSTAL STRUCTURE DETERMINATION AND REFINEMENT OF TWO CRYSTAL FORMS OF LIPASE B FROM CANDIDA ANTARCTICA

Experimental procedure

Spacegroup name	P 1 21 1
Unit cell lengths	67.000, 50.500, 86.700
Unit cell angles	90.00, 100.10, 90.00

Refinement procedure

Resolution	7.500 - 2.500
R-factor	0.196
Rwork	0.196
RMSD bond length	0.006
RMSD bond angle	1.300
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.500 *
Rmerge	0.056 *
Total number of observations	48771 *
Number of reflections	18925 *
Completeness [%]	94.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	3.6 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG4000	20 (%)
2	1	reservoir	sodium acetate	50 (mM)
3	1	reservoir	isopropanol	10 (%)
4	1	drop	protein	10 (mg/ml)
5	1	drop	beta-octyl glucoside	0.6 (%)