1TC6
Ligand Induced Conformational Shift in the N-terminal Domain of GRP94, Open Conformation ADP-Complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-04-02 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.000 |
Spacegroup name | H 3 2 |
Unit cell lengths | 159.234, 159.234, 109.070 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 1.870 |
R-factor | 0.225 |
Rwork | 0.225 |
R-free | 0.25900 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.940 |
High resolution limit [Å] | 1.870 | 1.870 |
Number of reflections | 43460 | |
<I/σ(I)> | 49.6 | 2.2 |
Completeness [%] | 99.7 | 100 |
Redundancy | 11.1 | 10.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | PEG400, MgCl, Tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |