1TC3

TRANSPOSASE TC3A1-65 FROM CAENORHABDITIS ELEGANS

Experimental procedure

Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7A
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7A
Temperature [K]	113
Detector technology	IMAGE PLATE
Collection date	1996-08-01
Detector	MARRESEARCH
Spacegroup name	C 2 2 21
Unit cell lengths	37.180, 202.790, 62.140
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.450
R-factor	0.234
R-free	0.31800
Structure solution method	MIRAS
RMSD bond length	0.013
RMSD bond angle	20.437 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASES
Refinement software	TNT (5-E)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.510
High resolution limit [Å]	2.450	2.450
Rmerge	0.055	0.438 *
Total number of observations	70943 *
Number of reflections	8089
<I/σ(I)>	19.4	1.93
Completeness [%]	89.7	67.1 *
Redundancy	8.8	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	4 *	PROTEIN/DNA COMPLEX WAS CRYSTALLIZED FROM 15% MPD, 100 MM NACL, 20 MM CACL2, 10 MM DTT, 50 MM NA ACETATE PH 5.5, VAPOR DIFFUSION, HANGING DROP

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	WATER
10	1	2	CACL2
11	1	2	DTT
12	1	2	NA ACETATE
2	1	1	MPD
3	1	1	NACL
4	1	1	CACL2
5	1	1	DTT
6	1	1	NA ACETATE
7	1	2	WATER
8	1	2	MPD
9	1	2	NACL

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir		100 (mM)
10	1	drop	sodium acetate	25 (mM)
11	1	drop	protein	2.25 (mg/ml)
2	1	reservoir		20 (mM)
3	1	reservoir	MPD	13-15 (%)
4	1	reservoir	dithiothreitol	10 (mM)
5	1	reservoir	sodium acetate	50 (mM)
6	1	drop		50 (mM)
7	1	drop		10 (mM)
8	1	drop		6.5-7.5 (%)
9	1	drop	dithiothreitol	5 (mM)