1TBR

CRYSTAL STRUCTURE OF INSECT DERIVED DOUBLE DOMAIN KAZAL INHIBITOR RHODNIIN IN COMPLEX WITH THROMBIN

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1994-01-10
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	114.735, 112.297, 92.069
Unit cell angles	90.00, 94.97, 90.00

Refinement procedure

Resolution	8.000 - 2.640 *
R-factor	0.189
Rwork	0.189
R-free	0.26200 *
RMSD bond length	0.010
RMSD bond angle	1.660
Data reduction software	MOSFLM
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	999.000
High resolution limit [Å]	2.600	2.600 *
Rmerge	0.049 *
Total number of observations	89438 *
Number of reflections	33844
Completeness [%]	94.5 *	78.2 *
Redundancy	2.64

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6 *	20 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG4000	10 (%)
2	1	reservoir	phosphate	300 (mM)