1TBL
H141N mutant of rat liver arginase I
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2001-10-01 |
Detector | MARRESEARCH |
Wavelength(s) | 0.98 |
Spacegroup name | P 32 |
Unit cell lengths | 88.332, 88.332, 113.417 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.560 - 3.100 |
R-factor | 0.298 |
Rwork | 0.298 |
R-free | 0.30600 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 |
RMSD bond angle | 1.600 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.000 | 3.200 |
High resolution limit [Å] | 3.100 | 3.100 |
Number of reflections | 18163 | |
Completeness [%] | 99.8 | 99.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | bicine, PEG8000, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |