1TAU
TAQ POLYMERASE (E.C.2.7.7.7)/DNA/B-OCTYLGLUCOSIDE COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-06-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 107.600, 107.600, 170.010 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 3.000 |
| Rwork | 0.244 |
| R-free | 0.30500 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 22.300 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 3.000 |
| Rmerge | 0.088 |
| Number of reflections | 21866 |
| <I/σ(I)> | 8.9 |
| Completeness [%] | 93.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 22 * | pH 6.00, VAPOR DIFFUSION, HANGING DROP, temperature 295.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | PEG 8000 | ||
| 3 | 1 | 1 | NA CITRATE | ||
| 4 | 1 | 1 | ZN SULFATE |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | sodium citrate | 100 (mM) | |
| 2 | 1 | drop | PEG8000 | 4 (%(w/v)) | |
| 3 | 1 | drop | 1 (mM) | ||
| 4 | 1 | drop | 0-400 (mM) |
