1TAR
CRYSTALLINE MITOCHONDRIAL ASPARTATE AMINOTRANSFERASE EXISTS IN ONLY TWO CONFORMATIONS
Experimental procedure
Spacegroup name | P 1 |
Unit cell lengths | 57.400, 59.400, 65.500 |
Unit cell angles | 83.10, 104.80, 83.30 |
Refinement procedure
Resolution | 8.000 - 2.200 |
R-factor | 0.194 |
RMSD bond length | 0.014 |
RMSD bond angle | 2.200 |
Refinement software | TNT |
Data quality characteristics
Overall | Outer shell | |
High resolution limit [Å] | 2.200 * | 2.200 * |
Rmerge | 0.069 * | |
Total number of observations | 44017 * | |
Number of reflections | 36893 * | |
Completeness [%] | 88.5 * | 84.1 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.5 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG4000 | 17-20 (%(w/v)) | |
2 | 1 | reservoir | ammonium acetate | 0.5 (M) | |
3 | 1 | reservoir | phosphate | 20 (mM) | pH7.5 |