1TAQ
STRUCTURE OF TAQ DNA POLYMERASE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 108 |
| Detector technology | IMAGE PLATE AREA DETECTOR |
| Collection date | 1994-09 |
| Detector | MARRESEARCH |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 107.421, 107.421, 170.245 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.000 - 2.400 |
| R-factor | 0.202 |
| Rwork | 0.202 |
| R-free | 0.32300 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.790 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.400 * |
| Rmerge | 0.046 |
| Number of reflections | 40493 * |
| Completeness [%] | 90.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8.2 * | 22 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 10 | 1 | reservoir | dithiothreitol | 2 (mM) | |
| 11 | 1 | reservoir | sodium azide | 0.2 (%(w/v)) | |
| 12 | 1 | reservoir | sodium citrate | 100 (mM) | |
| 2 | 1 | drop | Tris-HCl | 18 (mM) | |
| 3 | 1 | drop | EDTA | 0.09 (mM) | |
| 4 | 1 | drop | dithiothreitol | 0.9 (mM) | |
| 5 | 1 | drop | 90 (mM) | ||
| 6 | 1 | drop | glycerol | 9 (%(v/v)) | |
| 7 | 1 | drop | beta-octyl glucoside | 0.7 (%(w/v)) | |
| 8 | 1 | reservoir | PEG8000 | 15 (%(w/v)) | |
| 9 | 1 | reservoir | ammonium sulfate | 60 (mM) |
