1TAQ
STRUCTURE OF TAQ DNA POLYMERASE
Experimental procedure
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12C |
Synchrotron site | NSLS |
Beamline | X12C |
Temperature [K] | 108 |
Detector technology | IMAGE PLATE AREA DETECTOR |
Collection date | 1994-09 |
Detector | MARRESEARCH |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 107.421, 107.421, 170.245 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.000 - 2.400 |
R-factor | 0.202 |
Rwork | 0.202 |
R-free | 0.32300 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.790 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
High resolution limit [Å] | 2.400 * |
Rmerge | 0.046 |
Number of reflections | 40493 * |
Completeness [%] | 90.0 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8.2 * | 22 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
10 | 1 | reservoir | dithiothreitol | 2 (mM) | |
11 | 1 | reservoir | sodium azide | 0.2 (%(w/v)) | |
12 | 1 | reservoir | sodium citrate | 100 (mM) | |
2 | 1 | drop | Tris-HCl | 18 (mM) | |
3 | 1 | drop | EDTA | 0.09 (mM) | |
4 | 1 | drop | dithiothreitol | 0.9 (mM) | |
5 | 1 | drop | 90 (mM) | ||
6 | 1 | drop | glycerol | 9 (%(v/v)) | |
7 | 1 | drop | beta-octyl glucoside | 0.7 (%(w/v)) | |
8 | 1 | reservoir | PEG8000 | 15 (%(w/v)) | |
9 | 1 | reservoir | ammonium sulfate | 60 (mM) |