1TAH

THE CRYSTAL STRUCTURE OF TRIACYLGLYCEROL LIPASE FROM PSEUDOMONAS GLUMAE REVEALS A PARTIALLY REDUNDANT CATALYTIC ASPARTATE

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	158.160, 158.640, 63.360
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 3.000
R-factor	0.159
Rwork	0.159
R-free	0.22600
RMSD bond length	0.010
RMSD bond angle	2.500
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Rmerge	0.046 *
Total number of observations	92181 *
Number of reflections	25924 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10-20 (mg/ml)
2	1	drop	Tris	0.1 (M)
3	1	drop	acetone	10 (%(v/v))
4	1	drop	n-dodecyl-beta-D-glucopyranoside		trace amounts
5	1	resrvoir	PEG8000	27-29 (%)