1TAD
GTPASE MECHANISM OF GPROTEINS FROM THE 1.7-ANGSTROM CRYSTAL STRUCTURE OF TRANSDUCIN ALPHA-GDP-ALF4-
Experimental procedure
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Detector technology | IMAGE PLATE |
Collection date | 1993-02-11 |
Detector | FUJI |
Wavelength(s) | 0.95 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 74.900, 108.200, 79.000 |
Unit cell angles | 90.00, 111.70, 90.00 |
Refinement procedure
Resolution | 8.000 - 1.700 |
R-factor | 0.209 |
Rwork | 0.209 |
R-free | 0.26600 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | Outer shell | |
High resolution limit [Å] | 1.700 * | 1.700 * |
Rmerge | 0.103 * | 0.186 * |
Number of reflections | 116584 | 12535 * |
Completeness [%] | 88.0 | 76.7 * |
Redundancy | 3 | 4.22 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Batch method * | 6 * | 4 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | protein | 20 (mg/ml) | |
2 | 1 | 1 | PEG8000 | 20 (%(w/v)) | |
3 | 1 | 1 | 200 (mM) | ||
4 | 1 | 1 | sodium cacodylate | 100 (mM) | |
5 | 1 | 1 | beta-mercaptoethanol | 0.2 (%(v/v)) | |
6 | 1 | 1 | 5 (mM) | ||
7 | 1 | 1 | 0.1 (M) | ||
8 | 1 | 1 | 30 (mM) | ||
9 | 1 | 1 | glycerol | 40 (%(v/v)) |