1T9Z

Three-dimensional structure of a RNA-polymerase II binding protein.

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	CCD
Collection date	2003-12-03
Detector	MARRESEARCH
Wavelength(s)	0.9795, 0.9797, 0.9686
Spacegroup name	P 21 21 2
Unit cell lengths	117.550, 47.190, 39.980
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.300
Rwork	0.187
R-free	0.23400
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.290
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.380
High resolution limit [Å]	2.300	2.300
Number of reflections	9635
<I/σ(I)>		14
Completeness [%]	90.9	80.1
Redundancy	17

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	PEG 3300, ammonium acetate, citrate buffer, DTT , pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K