1T9Z
Three-dimensional structure of a RNA-polymerase II binding protein.
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-12-03 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9795, 0.9797, 0.9686 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 117.550, 47.190, 39.980 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.300 |
Rwork | 0.187 |
R-free | 0.23400 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.290 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 9635 | |
<I/σ(I)> | 14 | |
Completeness [%] | 90.9 | 80.1 |
Redundancy | 17 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | PEG 3300, ammonium acetate, citrate buffer, DTT , pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |