1T9H
The crystal structure of YloQ, a circularly permuted GTPase.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2003-10-11 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.870 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 47.970, 47.970, 279.890 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.500 - 1.600 |
| R-factor | 0.14565 |
| Rwork | 0.144 |
| R-free | 0.17801 |
| Structure solution method | MIR |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.253 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | DM |
| Refinement software | REFMAC (5.1.9999) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.127 | 0.500 |
| Number of reflections | 44626 | |
| <I/σ(I)> | 6.3 | 2.7 |
| Completeness [%] | 99.4 | 99.7 |
| Redundancy | 4.7 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | PEG 5000, calcium acetate, HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
