1T98
Crystal Structure of MukF(1-287)
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-01-03 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.1272, 0.9797 |
Spacegroup name | P 61 |
Unit cell lengths | 58.699, 58.699, 307.414 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 2.900 |
R-factor | 0.235 |
Rwork | 0.233 |
R-free | 0.27300 |
Structure solution method | MAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.141 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHARP |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 102.471 | 3.060 |
High resolution limit [Å] | 2.900 | 2.900 |
Number of reflections | 12713 | |
<I/σ(I)> | 12.25 | 4.5 |
Completeness [%] | 95.9 | 95.9 |
Redundancy | 5.3 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 291 | PEG 2K MME, calcium chloride, ammonium phosphate, bis-tris propane, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K, pH 8.50 |