1T92

Crystal structure of N-terminal truncated pseudopilin PulG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	SCHNEIDER
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2002-09-27
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 65 2 2
Unit cell lengths	85.395, 85.395, 145.720
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 1.600
R-factor	0.169
Rwork	0.167
R-free	0.19800
Structure solution method	MAD
RMSD bond length	0.012
RMSD bond angle	1.422
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELXD
Refinement software	REFMAC (5.1.25)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.650
High resolution limit [Å]	1.600	1.600
Number of reflections	40533
<I/σ(I)>	19.27	4.2
Completeness [%]	96.2	99.7
Redundancy	25.14	8.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2		HEPES, ammonium sulfate, isopropanol, sodium azide, pH 7.2, VAPOR DIFFUSION, HANGING DROP, SEEDING, temperature 100.0K
1	VAPOR DIFFUSION, HANGING DROP	7.2		HEPES, ammonium sulfate, isopropanol, sodium azide, pH 7.2, VAPOR DIFFUSION, HANGING DROP, SEEDING, temperature 100.0K