1T8S
Crystal Structure of E.coli AMP Nucleosidase complexed with formicin 5'-monophosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 8-BM |
Synchrotron site | APS |
Beamline | 8-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-04-04 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9778 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 246.240, 246.240, 111.130 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.750 - 2.600 |
R-factor | 0.226 |
Rwork | 0.226 |
R-free | 0.25600 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.760 |
High resolution limit [Å] | 2.600 | 2.600 |
Number of reflections | 100422 | |
<I/σ(I)> | 37.9 | 4 |
Completeness [%] | 95.8 | 100 |
Redundancy | 7.7 | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 291 | ammonium formate, sodium chloride, sodium citrate, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 291K |