1T8G

Crystal structure of phage T4 lysozyme mutant L32A/L33A/T34A/C54T/C97A/E108V

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-1
Synchrotron site	SSRL
Beamline	BL9-1
Temperature [K]	100
Spacegroup name	I 4 2 2
Unit cell lengths	96.037, 96.037, 77.265
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.370 - 1.800
R-factor	0.184
Rwork	0.184
R-free	0.21500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.400
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.400	1.850
High resolution limit [Å]	1.800	1.800
Number of reflections	16520
<I/σ(I)>	15.1	8.7
Completeness [%]	97.7	97.7
Redundancy	8.3	7.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1