1T8F

Crystal structure of phage T4 lysozyme mutant R14A/K16A/I17A/K19A/T21A/E22A/C54T/C97A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-1
Synchrotron site	SSRL
Beamline	BL9-1
Temperature [K]	100
Spacegroup name	P 62 2 2
Unit cell lengths	120.518, 120.518, 50.317
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.450 - 2.150
R-factor	0.224
Rwork	0.224
R-free	0.27500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.200
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.400	2.200
High resolution limit [Å]	2.150	2.150
Number of reflections	11852
<I/σ(I)>	5.1	1.6
Completeness [%]	97.6	83.3
Redundancy	5.2	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1