1T8B
Crystal structure of refolded PHOU-like protein (gi 2983430) from Aquifex aeolicus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-01-10 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9792 |
Spacegroup name | P 32 |
Unit cell lengths | 85.094, 85.094, 62.825 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 15.000 - 3.230 |
R-factor | 0.21596 |
Rwork | 0.214 |
R-free | 0.24846 |
Structure solution method | SAD + molecular replacement |
Starting model (for MR) | 1t72 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.422 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.000 | 3.393 |
High resolution limit [Å] | 3.230 | 3.230 |
Rmerge | 0.109 | 0.780 |
Number of reflections | 8109 | |
<I/σ(I)> | 14 | 2.1 |
Completeness [%] | 99.3 | 90 |
Redundancy | 12.2 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | Lithium Sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |