1T8B
Crystal structure of refolded PHOU-like protein (gi 2983430) from Aquifex aeolicus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-01-10 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 32 |
| Unit cell lengths | 85.094, 85.094, 62.825 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 3.230 |
| R-factor | 0.21596 |
| Rwork | 0.214 |
| R-free | 0.24846 |
| Structure solution method | SAD + molecular replacement |
| Starting model (for MR) | 1t72 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.422 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.000 | 3.393 |
| High resolution limit [Å] | 3.230 | 3.230 |
| Rmerge | 0.109 | 0.780 |
| Number of reflections | 8109 | |
| <I/σ(I)> | 14 | 2.1 |
| Completeness [%] | 99.3 | 90 |
| Redundancy | 12.2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | Lithium Sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
