1T7Z
Zn-alpha-2-glycoprotein; baculo-ZAG no PEG, no glycerol
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-09-28 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.0781 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 122.084, 122.084, 65.415 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 3.000 |
Rwork | 0.217 |
R-free | 0.26800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1t7v |
RMSD bond length | 0.007 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.065 | 0.182 |
Number of reflections | 9940 | |
<I/σ(I)> | 16.6 | 5.7 |
Completeness [%] | 96.9 | 79.4 |
Redundancy | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 7.5 | 298 | Ammonium sulfate, trehalose, HEPES, pH 7.5, Microbatch, temperature 298.0K |