1T71
Crystal structure of a novel phosphatase Mycoplasma pneumoniaefrom
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2003-06-03 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 84.896, 84.896, 162.726 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.960 - 2.100 |
R-factor | 0.2151 |
Rwork | 0.215 |
R-free | 0.24700 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | EPMR |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.200 | 2.140 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 31951 | |
Completeness [%] | 89.7 | 56.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | Bis-Tris, AMS, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |