1T6Z
Crystal structure of riboflavin bound TM379
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2001-11-10 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.00000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 66.535, 81.943, 66.709 |
Unit cell angles | 90.00, 116.96, 90.00 |
Refinement procedure
Resolution | 19.370 - 2.400 |
R-factor | 0.2251 |
Rwork | 0.225 |
R-free | 0.29500 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.500 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 24508 | |
Completeness [%] | 95.0 | 89.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | PEG 4000, sodium citrate, glycerol, propanol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |