1T6N
Crystal structure of the N-terminal domain of human UAP56
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-10-10 |
Wavelength(s) | 0.97921, 0.97923, 0.96417 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 49.453, 47.284, 87.190 |
Unit cell angles | 90.00, 98.23, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.940 |
R-factor | 0.216 |
Rwork | 0.216 |
R-free | 0.25500 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.340 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.010 |
High resolution limit [Å] | 1.940 | 1.940 |
Rmerge | 0.060 | 0.450 |
Number of reflections | 29132 | |
<I/σ(I)> | 25.5 | 5.4 |
Completeness [%] | 98.7 | |
Redundancy | 5.1 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 291 | sodium citrate, PEG MME 2000, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 291 | sodium citrate, PEG MME 2000, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 291 | sodium citrate, PEG MME 2000, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |