1T6L
Crystal Structure of the Human Cytomegalovirus DNA Polymerase Subunit, UL44
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12C |
Synchrotron site | NSLS |
Beamline | X12C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-07-23 |
Detector | BRANDEIS - B4 |
Wavelength(s) | 0.9786, 0.9788, 0.9500 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 53.930, 53.930, 340.067 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 12.000 - 1.850 |
R-factor | 0.23831 |
Rwork | 0.235 |
R-free | 0.26800 |
Structure solution method | MAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.577 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.930 |
High resolution limit [Å] | 1.850 | 1.850 |
Number of reflections | 26592 | |
<I/σ(I)> | 58.4 | 14.2 |
Completeness [%] | 99.8 | 100 |
Redundancy | 28.8 | 18 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 295 | polyethylene glycol 4000, sodium acetate, sodium chloride, dithiothreitol , pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 295 | polyethylene glycol 4000, sodium acetate, sodium chloride, dithiothreitol , pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 295 | polyethylene glycol 4000, sodium acetate, sodium chloride, dithiothreitol , pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 295K |