1T6F
Crystal Structure of the Coiled-coil Dimerization Motif of Geminin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-06-17 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54180 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 25.120, 43.440, 67.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.470 |
| R-factor | 0.1806 |
| Rwork | 0.179 |
| R-free | 0.22103 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1E7T |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.151 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | EPMR |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.520 | 1.520 |
| High resolution limit [Å] | 1.440 | 1.440 |
| Number of reflections | 12978 | |
| Completeness [%] | 92.8 | 88.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 10% PEG 6000, 5% MPD, 100 mM Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
