1T6A
Crystal Structure of Protein of Unknown Function from Bacillus stearothermophilus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-04-12 |
Detector | SBC-2 |
Wavelength(s) | 0.97952 |
Spacegroup name | H 3 2 |
Unit cell lengths | 77.307, 77.307, 200.410 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.000 - 2.050 |
R-factor | 0.19618 |
Rwork | 0.194 |
R-free | 0.22462 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.232 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SHELXD |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.100 |
High resolution limit [Å] | 2.050 | 2.050 |
Rmerge | 0.088 | 0.432 |
Number of reflections | 14757 | |
<I/σ(I)> | 22.4 | 2.07 |
Completeness [%] | 99.3 | 94.3 |
Redundancy | 9.9 | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 294 | Bis-Tris-Propane, Sodium Nitrate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 294K |