1T62
Crystal structure of protein EF3133 from Enterococcus faecalis V583, Pfam DUF984
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X9A |
Synchrotron site | NSLS |
Beamline | X9A |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2004-01-26 |
Detector | MARRESEARCH |
Wavelength(s) | 1.0083 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 88.250, 88.250, 106.001 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.000 - 3.000 |
R-factor | 0.219 |
Rwork | 0.215 |
R-free | 0.26300 |
Structure solution method | SIRAS |
RMSD bond length | 0.008 |
RMSD bond angle | 1.350 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 3.100 |
High resolution limit [Å] | 3.000 | 3.000 |
Number of reflections | 9754 | |
Completeness [%] | 98.4 | 92 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | PEG 2000, Succinic Acid, HEPES, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |