1T5Y

Crystal Structure of Northeast Structural Genomics Consortium Target HR2118: A Human Homolog of Saccharomyces cerevisiae Nip7p

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2004-04-02
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97895
Spacegroup name	P 41 21 2
Unit cell lengths	58.030, 58.030, 132.690
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.600 - 2.500
R-factor	0.213
Rwork	0.213
R-free	0.29400
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.142	0.603
Number of reflections	14942
<I/σ(I)>	5.9
Completeness [%]	99.9	99.5
Redundancy	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.8		200 mM magnesium sulfate, 20% PEG, 100 mM sodium acetate, 10 mM DTT, pH 5.8, VAPOR DIFFUSION, HANGING DROP