1T5I
Crystal structure of the C-terminal domain of UAP56
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-10-10 |
Wavelength(s) | 0.97934, 0.97949, 0.96411 |
Spacegroup name | P 43 |
Unit cell lengths | 33.582, 33.582, 119.075 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.900 |
Rwork | 0.224 |
R-free | 0.26780 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.343 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.060 | 0.258 |
Number of reflections | 9977 | |
<I/σ(I)> | 20.8 | 2.8 |
Completeness [%] | 95.8 | 72 |
Redundancy | 3.6 | 1.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | MOPS, NaCl, Sodium Phosphate, Potassium Phosphate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | MOPS, NaCl, Sodium Phosphate, Potassium Phosphate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |