1T5B
Structural genomics, A protein from Salmonella typhimurium similar to E. coli acyl carrier protein phosphodiesterase
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-02-25 |
Detector | SBC-2 |
Wavelength(s) | 0.9795,0.9798,0.94656 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 40.891, 107.915, 51.168 |
Unit cell angles | 90.00, 111.45, 90.00 |
Refinement procedure
Resolution | 30.550 - 1.400 |
R-factor | 0.196 |
Rwork | 0.196 |
R-free | 0.22300 |
Structure solution method | MAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.700 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.450 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.038 | 0.165 |
Number of reflections | 164570 | |
<I/σ(I)> | 32.07 | 4.63 |
Completeness [%] | 96.3 | |
Redundancy | 3.4 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 10% Glycerol, 20.5% PEG 4000, 0.065 M tris-sodium Citrate, 0.1 M NH4 acetate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |