1T57
Crystal Structure of the Conserved Protein MTH1675 from Methanobacterium thermoautotrophicum
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-06-20 |
Detector | SBC-2 |
Wavelength(s) | 0.9794, 0.9795, 0.956 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 103.983, 69.398, 90.953 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.920 - 2.300 |
R-factor | 0.259 |
Rwork | 0.252 |
R-free | 0.31800 |
Structure solution method | MAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.800 |
Data reduction software | d*TREK |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.064 | 0.538 |
Number of reflections | 28562 | |
<I/σ(I)> | 8.8 | 1.8 |
Completeness [%] | 95.7 | 93.6 |
Redundancy | 5.5 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | HEPES, magnesium acetate, PEG 3350, Glycerol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | HEPES, magnesium acetate, PEG 3350, Glycerol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | HEPES, magnesium acetate, PEG 3350, Glycerol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |