1T4G
ATPase in complex with AMP-PNP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ENRAF-NONIUS |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-01-16 |
Detector | ENRAF-NONIUS |
Wavelength(s) | 1.5418 |
Spacegroup name | P 61 |
Unit cell lengths | 83.699, 83.699, 106.672 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 2.000 |
R-factor | 0.219 |
Rwork | 0.219 |
R-free | 0.24400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1pzn |
RMSD bond length | 0.006 |
RMSD bond angle | 1.220 |
Data reduction software | LSCALE |
Data scaling software | LSCALE |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 1.800 |
Number of reflections | 38530 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.2M magnesium chloride, 0.1M Tris, 6% PEG 3350, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |